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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Nat Protoc. 2015 Jul 23;10(8):1264–1274. doi: 10.1038/nprot.2015.080

Figure 4.

Figure 4

Macroscopic appearance of primary HCC and lung metastases. (a) HCA-1 grafted in cirrhotic liver (after 12 weeks of CCl4 treatment). Scale bar, 10 mm. (b) HCCs forming after adeno-Cre injection in Stk4−/−Stk3F/− mice with liver cirrhosis (after 12 weeks of CCl4 treatment). Scale bar, 10 mm. (c) Primary HCC and lung metastases in healthy (top), fibrotic (middle) and cirrhotic (bottom) livers in the orthotopic HCA-1 graft model. Scale bar, 10 mm. Institutional regulatory board permission from MGH IACUC was obtained for all procedures performed within this protocol.