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. Author manuscript; available in PMC: 2016 Mar 21.
Published in final edited form as: Nature. 2015 May 4;522(7556):368–372. doi: 10.1038/nature14352

Figure 4. Application of PLOR-generated constructs for smFRET.

Figure 4

a, Secondary structure diagram of riboA71 depicting the locations of the fluorescent labels. b, smFRET probability histogram for free diffusion experiments conducted in solutions containing 1 mM Mg2+ and either 0 or 100 μM adenine. c, Ligand binding curve for riboA71. d, False-color image of surface-immobilized molecules reveals that RNA constructs contain all of the specified modifications and are able to form the high-FRET conformation. e, Single-step photobleaching confirms the existence of a single donor-acceptor FRET pair.