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. Author manuscript; available in PMC: 2016 Mar 21.
Published in final edited form as: Nature. 2015 May 4;522(7556):368–372. doi: 10.1038/nature14352

Extended Data Figure 3. Optimization of experimental conditions for PLOR synthesis.

Extended Data Figure 3

a, The products of the PLOR (left) and the standard transcription methods (right). PLOR generates a pure full-length product with the desired labeling in the final step. b, Comparison of PLOR efficiency for Lp2-CN synthesis under various conditions: (I) freshness of DNA-attached beads, (II) anion specificity, (III) [Mg2+], (IV) K2SO4 presence in the initiation, (V) increasing NTPs (1X represents NTP amounts in Extended Data Table 1), (VI) ratio of ATP/GTP:DNA, (VII) ratio of UTP:DNA, and (VIII) incubation time of the termination. The right lane contains pure RiboA71 as a control.