Extended Data Figure 4. Comparison of NMR spectra of PLOR-generated and 71nt-CN samples in adenine-bound form.
Superposition of the 1H13C-TROSY spectra of 71nt-CN with Lp1-CN (a), Lp2-CN (b), Lp1+2-CN (c), Lk2-CN (d), S1+Lk1-CN (e), and 4nt-CN (f). These results indicate that the RNAs synthesized by PLOR have the same fold as 71nt-CN generated using the traditional solution-based transcription method and are functional as evidenced by binding of the adenine ligand.