Extended Data Figure 5. PLOR-generated riboA71 maintains both sequence and structural fidelity.

a, Structural superposition of the PLOR-generated riboA71 (PDB::4XNR) with that of the riboA71 generated using the regular in vitro transcription (PDB: 4TZX)²⁶. The RMSD between all C1 atoms was ~ 0.3 Å. b, Structural superposition of the PLOR-generated riboA71 (PDB: 4XNR) with that of the riboA71 (PDB: 1Y26)¹. The RMSD between all C1 atoms was ~ 0.4 Å. c, Sequences and secondary structures of the RNAs in (b). The arrows denote nucleotide sequence differences between the two ribA71 sequences. d, Composite simulated anneal-omit 2|Fo|-|Fc| electron density of the riboA71 RNA structure (PDB: 4XNR) at 2.2 Å resolution calculated using the final model (1.0 s.d.) e, Portion of the electron density in (c) in the adenine binding pocket unambiguously identifies the nucleobase identities of the PLOR-generated RNA, revealing no undesired sequence changes introduced by PLOR. f, Portion of the electron density in (c) of the G6•C66 base pair which differs in identity between the two structures, suggesting that if there had been undesired sequence substitutions that resulted from using the PLOR method, they would have been readily detected in the crystallographic analysis.