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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Mol Cell Cardiol. 2015 Oct 19;89(Pt B):287–296. doi: 10.1016/j.yjmcc.2015.10.018

Figure 3.

Figure 3

Wnt activation by cardiac APC deletion promotes CM proliferation at E13.5. A to G, Double labeling of APC with β-catenin in WT (A-C) and Apc (D-F) deleted hearts (cKO) shows effective deletion of Apc and upregulation of β-catenin. G, Average β-catenin intensity is increased in Apc cKO hearts. H, Average APC intensity is decreased in Apc cKO hearts. I, Real time RT-PCR reveals downregulation of Apc and upregulation of β-catenin mRNA levels in Apc cKO heart. J, Cardiac morphology shows that Apc cKO mice have bigger hearts with thicker left (L) and right (R) ventricular walls compared to WT controls; Boxed regions are zoomed in adjacent images; Scale bars, 1 mm (white and, red) and 100 μm (black). K, Ventricular wall thickness (upper panel) in Apc cKO and WT mice and percentage of Ki67 positive CMs (lower panel) in ventricular wall of cKO and WT mice. L, Double labeling of Ki67 with Actinin. Data represent mean ± SD. N=3 independent experiments.