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(A) Intensity of collagen fibers was analyzed using ImageJ v1.33 and the Color Histogram plug-in. (B) Micrograph of the skin section stained with Masson trichrome staining (×100, (insertion, ×400)). Collagen fiber stained blue. Keratin, cytoplasm, and muscle stained red Abbreviation: SC, stratum corneau; E, viable epidermis; DS, dermis and subcutis.
