Table 1. Thermodynamic parameters of model β-D-RNA and β-L-RNA duplexes and hairpinsa.
| Duplexes (5ʹ–3ʹ) | Average of curve fits | TM−1 vs. log CT plots | ||||||
|---|---|---|---|---|---|---|---|---|
| −ΔH° (kcal/mol) | −ΔS° (eu) | −ΔG°37 (kcal/mol) | TM b (°C) | −ΔH° (kcal/mol) | −ΔS° (eu) | −ΔG°37 (kcal/mol) | TMb (°C) | |
| GUCGAC | 57.7±2.5 | 161.7±7.5 | 7.54±0.16 | 47.4 | 52.3±2.2 | 144.6±6.9 | 7.41±0.05 (7.09) | 47.7 (45.4) |
| GUCGAC | 58.1±3.7 | 162.8±11.3 | 7.59±0.17 | 47.6 | 51.1±1.2 | 140.7±3.9 | 7.43±0.02 | 48.0 |
| GACGUC | 58.8±2.4 | 164.5±7.5 | 7.77±0.07 | 48.4 | 57.9±1.4 | 161.8±4.4 | 7.72±0.03 (7.35) | 48.3 (46.2) |
| GACGUC | 59.5±3.3 | 166.6±10.3 | 7.82±0.12 | 48.6 | 56.5±0.7 | 157.4±2.3 | 7.70±0.02 | 48.5 |
| GCAUGC | 60.4±2.6 | 169.1±8.1 | 7.91±0.13 | 48.9 | 53.9±1.3 | 148.9±4.0 | 7.75±0.03 (7.38) | 49.4 (45.7) |
| GCAUGC | 60.5±1.7 | 169.4±5.2 | 7.91±0.10 | 48.9 | 57.5±1.3 | 160.3±4.0 | 7.82±0.03 | 49.0 |
| GUGCAC | 56.5±3.0 | 156.6±9.6 | 7.87±0.09 | 49.6 | 57.0±1.3 | 158.4±4.0 | 7.86±0.04 (7.65) | 49.3 (47.6) |
| GUGCAC | 57.1±2.1 | 158.5±6.5 | 7.90±0.14 | 49.6 | 51.3±1.0 | 140.5±3.2 | 7.76±0.03 | 50.1 |
| CGUGCGAAUGAACGCACGH | 58.6±1.3 | 167.5±3.9 | 6.65±0.12 | 76.7 | ||||
| CGUGCGAAUGAACGCACGH | 58.8±2.2 | 168.2±6.9 | 6.61±0.10 | 76.3 | ||||
| GGCGCAAGCCH | 35.3±7.2 | 103.0±21.2 | 3.38±0.75 | 69.8 | ||||
| GGCGCAAGCCH | 37.3±6.1 | 109.4±18.9 | 3.40±0.28 | 68.0 | ||||
a–solutions: 1 M NaCl, 20mM sodium cacodylate, 0.5 mM Na2EDTA, pH 7;
H– 100 mM NaCl, 20 mM sodium cacodylate, 0.5 mM Na2EDTA, pH 7;
b–calculated for 10−4 M oligomer concentration,
italic– β-L-RNA
values in parentheses–results from PNAS USA (1986), 83, 9373.