Figure 5. Meta analysis of complex LSVs.
(A) Fold enrichment (green dots) of complex LSVs calculated by comparing the fraction of complex LSVs among differentially spliced LSVs (dark blue bars) to their relative proportion (light blue bars) in 32 datasets. The corrected p-value column on the left measures significance of the fold enrichment (binomial test, Bonferroni corrected p-value) Medians are displayed for fold enrichment (green line, 1.63), fraction of complex LSVs among changing LSVs (orange line, 0.52), and fraction of complex LSVs among all detected LSVs (red line, 0.31). Human AD versus healthy brain data corresponds to the cohort from (Bai et al., 2013). See Figure 5—source data 1 for more information. (B) Empirical cumulative distribution function (CDF) of the maximal change of junction inclusion ( ΔΨ ) across all mouse datasets in Figure 5A. Only the LSVs detected in the twelve mouse tissues (Figure 4) are included. The plot includes junctions in binary LSVs (grey), and the second, third, and least changing junction in complex LSVs (light, medium, dark green). Dashed vertical line denotes ΔΨ of 10%. (C) Per nucleotide average conservation score (phastCons60 track) in regions proximal to single source (top) and single target (bottom) LSVs that were differentially spliced between any pair of tissues shown in Figure 4. The average is plotted for the subsets of complex (green) LSVs and binary (grey) LSVs as well as around a randomly selected set of constitutively spliced junctions (red, see Materials and methods for details).
DOI: http://dx.doi.org/10.7554/eLife.11752.011
Figure 5—figure supplement 1. Empirical cumulative distribution function (CDF) of the maximal junction inclusion (E[Ψ]) across all mouse datasets in Figure 5A.
