TABLE 1.
Primary Antibody Information1
| Antigen | Immunizing antigen | Manufacturer details | Dilution |
|---|---|---|---|
| Glutamic acid decaboxylase-67 (GAD67) |
Recombinant GAD67 protein corresponding to amino acids 4–10 of human GAD67 |
Chemicon (Millipore), catalog No. MAB5406, mouse monoclonal |
1:1,000 |
| Neuronal nuclear protein (NeuN) | Cell nuclei purified from mouse brain | Chemicon (Millipore), catalog No. MAB377, mouse monoclonal |
1:10,000 |
| Neuropeptide Y (NPY) | Synthetic porcine peptide (whole molecule) conjugated to KLH |
Sigma-Aldrich, catalog No. N9528, rabbit polyclonal |
1:4,000 |
| Somatostatin (SOM) | Synthetic peptide LELQRSANSNPAMAPRERK corresponding to amino acids 84–102 of human SOM |
Santa Cruz Biotechnology, catalog No. SC7819, goat polyclonal |
1:400 |
1
Concentrations listed indicate the optimal antibody dilutions used in each experiment.