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. Author manuscript; available in PMC: 2016 Mar 21.
Published in final edited form as: Ultramicroscopy. 2008 Aug 6;109(1):22–31. doi: 10.1016/j.ultramic.2008.07.007

Figure 2.

Figure 2

Membrane features associated with cell movement such as filopodia, and fine structures in the leading and trailing edges. [A] and [B] represent AFM topographies and the corresponding deflection images, respectively. BMMCs were stained with CFSE, sensitized overnight with anti-DNP-IgE and then spun onto a glass coverslip coated with DNP-BSA. After 30 min incubation, cells were fixed with 3.7% formaldehyde for 30 min. [C] is a 3D rendering of the topographic image. Cursor profiles crossing the filopodia shown in [A] and [B] are shown in the left column to provide corresponding height data at a given cursor line. AFM images of the fixed cell was conducted in contact mode with CSC38 cantilevers (k = 0.03 N/m, MikroMasch lever B).