Fig 3. Recruitment of ERα to the TFPI 5’-flanking region in vivo.

ChIP assays using anti-ERα antibodies were performed on chromatin isolated from MCF7 cells cultured in phenol red-free medium in the absence or presence of 10 nM EE2 for 4 hours. The equivalent fraction of the sonicated chromatin was set aside as 'input' DNA (non-immunoprecipitated) before the antibody affinity manipulations. The immunoprecipitated DNA and input was analyzed by conventional PCR with primers covering the ERE half-sites. For negative control, primers spanning a region without ERE half-sites were used. One representative result from two independent experiments is shown.