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. 2016 Apr 1;1440:135–144. doi: 10.1016/j.chroma.2016.02.057

Fig. 3.

Fig. 3

Effect of pore size on the analysis of dsDNA/RNA using IP RP HPLC. IP RP HPLC chromatograms of the analysis pUC18 HaeIII digest analysed using superficially porous particles. (A) 80 Å pore size using TEAA gradient 5. (B) 150 Å pore size using TEAA gradient 5 and (C) 400 Å pore size using TEAA gradient 6. 250 ng of pUC18 HaeIII digest was analysed at 50 °C, UV detection at 260 nm. (D) IP RP HPLC chromatogram of the MS2 RNA on 400 Å pore size superficially porous particles using TEAA gradient 7.50 ng MS2 RNA was injected and analysed at 50 °C UV detection at 260 nm.