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. 2016 Mar 21;11(3):e0152138. doi: 10.1371/journal.pone.0152138

Fig 2. Effect of Tamoxifen and ICI 182,780 on basal forskolin-stimulated cAMP levels in mHippoE-18 cells.

Fig 2

Cells were pre-incubated with 100 μM IBMX for 20 min, followed by incubation with 10 μM forskolin and 100 μM IBMX in the presence of increasing concentrations of the various agonists for a further 20 min. The basal level in the absence of agonist is shown as 100%. Values are significantly different from basal as follows: Tamoxifen, 1 nM p <0.01, 3 nM p <0.005, 10 nM p <0.005; ICI 182,780, 3 nM p <0.05, 10 nM p <0.05, 30 nM p <0.01, 100 nM p <0.005, 300 nM p <0.05. Data are expressed as the mean ± SEM. n ≥ 3.