Fig 3. CD44 positivity of eosinophils, CD4⁺ and CD8⁺ T lymphocytes of WT C57BL/6 mice exposed to HDM and CS for 3 weeks.

A: Gating strategy for identifying eosinophils. CD11c^low, CD11b ^high, Siglec-F^high cells are identified as eosinophils. They are further characterized as being CD44^high. B: Gating strategy for identifying CD4⁺ and CD8⁺ T lymphocytes. T cells are identified as CD3^positive cells with low side scatter. They are further gated as either CD4^high or CD8^high. Both CD4⁺ and CD8⁺ T lymphocytes are CD44^positive.