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. 2016 Mar 14;113(11):3048–3053. doi: 10.1073/pnas.1517719113

Fig. 4.

Fig. 4.

SARS-CoV monoclonal antibodies have robust neutralization against WIV1 spike-mediated infection. Neutralization efficacy was evaluated using percent neutralization assays against SARS-CoV Urbani (black) or WIV1-MA15 (blue) with a panel of monoclonal antibodies: (A) fm6, (B) 230.15, (C) 227.15, and (D) 109.8, all originally generated against epidemic SARS-CoV. Each data point is representative of two or more independent neutralization wells. (E and F) Twenty- to twenty-four-week-old HFH4 ACE2-expressing mice were injected with 200 μg of anti-SARS human antibody 227.15 (hatched line) or mock (solid line) 1 d before infection with 1 × 10^5 pfu of SARS-CoV Urbani (black) or WIV1-CoV (blue) and examined over a 7-d time course for (E) survival (n = 3 for both antibody-treated groups and mock PBS control WIV1-CoV, n = 2 for mock-treated SARS-CoV Urbani), (F) day-2 lung titer (n = 3 for all groups). ND signifies no titers detected. For each bar graph, center value is representative of group mean and error bars are defined by SEM.