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Epigenetic border at the proviral CTCF-BS in fresh PBMCs of patients with ATL. (A–F) Distribution of H3K4me3, H3K36me3, H3K9Ac, and H2A.Z over the HTLV-1 provirus in ATL cells was analyzed by ChIP assay. The results are shown as the percentage of input DNA. Immunoprecipitated DNA was analyzed by real-time PCR using the primer sets as shown in Fig. 1A. N.A., not amplified in qPCR assay of input DNA, indicating that the ATL cells had a defective provirus.
