Fig. S1.

Multiple sequence alignment of the N-terminal nucleotide-binding domain of TRAP1 (mitochondria), HtpG (eubacteria), Hsp90 (cytosolic), and Grp94 (endoplasmic reticulum) from Homo sapiens (Hs), Danio rerio (Dr), E. coli (Ec), and S. cerevisiae (Sc). Residues conserved across sequences of representative members are highlighted in green, and those that are similar are in yellow. Residues not found in the mature protein are shown in lowercase letters. The N strap, ATP lid, and G1 and G2 boxes are marked. Residues that define the G1 and G2 box motifs are in red. Asp158, Phe183, Gln200, and Phe201 are surrounded by a gray box.