Fig. S7.

Chromatin effects of HDAC inhibition are dependent on HDAC-mediated transcriptional changes. (A) Endogenous EWSR1-FLI1 protein levels were measured in EWS894 cells after treatment with HDAC inhibitors (10 µM) by immunoblotting. (B) FAIRE-qPCR EWS502 cells with exogenous EWSR1-FLI1 treated with 10 μM Panobinostat. EWSR1-FLI1 targets sites were P1 and P7, and control FAIRE site was AURKAIP1. All treatments were for 16 h. Error bars represent the SD of three biological replicates (A) or three technical replicates (B).