AURKAIP1 forward (positive control) |
TATACCCGCAGGTCCAGAATCGTT |
AURKAIP1 reverse (positive control) |
AATAGCTCTAGACGCTTCCGCCTT |
BC006361 forward (negative control) |
TTCTCCAACTTTGGAAGCCCAGGA |
BC006361 reverse (negative control) |
TGTCTCCTTCTAGGCCCTCACAAT |
P1 forward (EWSR1-FLI1 binding) |
AAGGAAGGAAGGGAGGGACACATAC |
P1 reverse (EWSR1-FLI1 binding) |
CCTGTGAGTGTGACAGATTACTTGG |
P7 forward (EWSR1-FLI1 binding) |
GGGTGACAGAGTAAGATCCTGTCAGA |
P7 reverse (EWSR1-FLI1 binding) |
TGGGCGTGGTTCTCATGT |
Enhancer1 forward |
TCCTGACTCACTGCCTGGAGCAT |
Enhancer1 reverse |
GGGATTCCTGGAGTCACGGCA |
Enhancer2 forward |
GGCTCAAGCGCCTGGGTTTTGTA |
Enhancer2 reverse |
CAGCGTGGCTTCCTGGGCTTC |
Enhancer3 forward |
TTGCAAGTGAGTAAGCAGAAGCCCA |
Enhancer3 reverse |
AGCTGACCTCTCCCCTGTGTTAGT |
Enhancer4 forward |
AGTGTGGCGCCGCTCTGGTT |
Enhancer4 reverse |
CGTATTTGGCCACAGGAGGGAGC |
Enhancer5 forward |
ACGGCTGATCGTGTGACTTTCTGC |
Enhancer5 reverse |
GCCTTGACCAACTTGCTGAGGAGT |
Negative1 forward |
GTGGGAAGCTATGGCTGAGAAGCA |
Negative1 reverse |
TTGCCTTGGATTCCGTGCAGC |
Negative2 forward |
ACAGCCGGGGCAACTAGAAGA |
Negative2 reverse |
GACACCGCTTGGACCCGAGG |
Negative3 forward |
CCAGCTTGTCATGGCTACAGGGC |
Negative3 reverse |
GCTCACCGCAGTGACTCAGCAG |
Negative4 forward |
AGGTCAGCGTCAAAGAGGGGCT |
Negative4 reverse |
ACCTGACGCATGAAGGGCAGC |
Negative5 forward |
AGAGCCAAACCCAAGGAGGTAACTA |
Negative5 reverse |
GCCCACATGCATGCAATCCCA |
Promoter1 forward |
CGGGAGGCCAATCGAACGCC |
Promoter1 reverse |
GCTGAACGTCGCTCCTGACCC |
Promoter2 forward |
GCACCTCCCTCTGCCGCTTC |
Promoter2 reverse |
GCCCTCAAAGGGGCGGAACC |
Promoter3 forward |
ACTGCGTCGCTGGCTTGGAG |
Promoter3 reverse |
AAACGCGTGCCCAGCCAATCA |
Promoter4 forward |
GGAACAGATGAGGCAGGCAGGG |
Promoter4 reverse |
TTCTTTCCTCCTCTTTTTGCGGTGG |
Promoter5 forward |
GTGAGAGTGCAGGGCCCCAAG |
Promoter5 reverse |
GCCCGCCTTTCTCGCCGGTA |
Promoter6 forward |
TCACATGACCCGCCCAACCG |
Promoter6 reverse |
CGACGGCGGAAGAGAACGCT |
Target 3 forward |
GCATCAGGAAGCCTGGATCCATTA |
Target 3 reverse |
GTATATACCAACACCCTTCCCTG |
Target 4 forward |
AGATCCGGTTCAAATGGCAAGAGC |
Target 4 reverse |
GCACTCATCCTTAAGCCTCAACCA |
Control 2 forward |
CAAACTTCGGCTCACTTCGGCAAT |
Control 2 reverse |
AAGAAAGCCGAAACATGTCGCTCC |
EWSR1-FLI1 mRNA forward |
GCTATGGTCAACAAAGCAGCTATG |
EWSR1-FLI1 mRNA reverse |
TTGGCTAGGCGACTGCTGGT |
RPL27 mRNA forward |
GACGCAAAGCTGTCATCGTG |
RPL27 mRNA reverse |
GCAGTTTCTGGAAGAACCAC |