Skip to main content
. 2016 Feb 29;113(11):3018–3023. doi: 10.1073/pnas.1521827113

Table S3.

Primers used for FAIRE- and RNA-quantitative PCR

Name Oligo sequence
AURKAIP1 forward (positive control) TATACCCGCAGGTCCAGAATCGTT
AURKAIP1 reverse (positive control) AATAGCTCTAGACGCTTCCGCCTT
BC006361 forward (negative control) TTCTCCAACTTTGGAAGCCCAGGA
BC006361 reverse (negative control) TGTCTCCTTCTAGGCCCTCACAAT
P1 forward (EWSR1-FLI1 binding) AAGGAAGGAAGGGAGGGACACATAC
P1 reverse (EWSR1-FLI1 binding) CCTGTGAGTGTGACAGATTACTTGG
P7 forward (EWSR1-FLI1 binding) GGGTGACAGAGTAAGATCCTGTCAGA
P7 reverse (EWSR1-FLI1 binding) TGGGCGTGGTTCTCATGT
Enhancer1 forward TCCTGACTCACTGCCTGGAGCAT
Enhancer1 reverse GGGATTCCTGGAGTCACGGCA
Enhancer2 forward GGCTCAAGCGCCTGGGTTTTGTA
Enhancer2 reverse CAGCGTGGCTTCCTGGGCTTC
Enhancer3 forward TTGCAAGTGAGTAAGCAGAAGCCCA
Enhancer3 reverse AGCTGACCTCTCCCCTGTGTTAGT
Enhancer4 forward AGTGTGGCGCCGCTCTGGTT
Enhancer4 reverse CGTATTTGGCCACAGGAGGGAGC
Enhancer5 forward ACGGCTGATCGTGTGACTTTCTGC
Enhancer5 reverse GCCTTGACCAACTTGCTGAGGAGT
Negative1 forward GTGGGAAGCTATGGCTGAGAAGCA
Negative1 reverse TTGCCTTGGATTCCGTGCAGC
Negative2 forward ACAGCCGGGGCAACTAGAAGA
Negative2 reverse GACACCGCTTGGACCCGAGG
Negative3 forward CCAGCTTGTCATGGCTACAGGGC
Negative3 reverse GCTCACCGCAGTGACTCAGCAG
Negative4 forward AGGTCAGCGTCAAAGAGGGGCT
Negative4 reverse ACCTGACGCATGAAGGGCAGC
Negative5 forward AGAGCCAAACCCAAGGAGGTAACTA
Negative5 reverse GCCCACATGCATGCAATCCCA
Promoter1 forward CGGGAGGCCAATCGAACGCC
Promoter1 reverse GCTGAACGTCGCTCCTGACCC
Promoter2 forward GCACCTCCCTCTGCCGCTTC
Promoter2 reverse GCCCTCAAAGGGGCGGAACC
Promoter3 forward ACTGCGTCGCTGGCTTGGAG
Promoter3 reverse AAACGCGTGCCCAGCCAATCA
Promoter4 forward GGAACAGATGAGGCAGGCAGGG
Promoter4 reverse TTCTTTCCTCCTCTTTTTGCGGTGG
Promoter5 forward GTGAGAGTGCAGGGCCCCAAG
Promoter5 reverse GCCCGCCTTTCTCGCCGGTA
Promoter6 forward TCACATGACCCGCCCAACCG
Promoter6 reverse CGACGGCGGAAGAGAACGCT
Target 3 forward GCATCAGGAAGCCTGGATCCATTA
Target 3 reverse GTATATACCAACACCCTTCCCTG
Target 4 forward AGATCCGGTTCAAATGGCAAGAGC
Target 4 reverse GCACTCATCCTTAAGCCTCAACCA
Control 2 forward CAAACTTCGGCTCACTTCGGCAAT
Control 2 reverse AAGAAAGCCGAAACATGTCGCTCC
EWSR1-FLI1 mRNA forward GCTATGGTCAACAAAGCAGCTATG
EWSR1-FLI1 mRNA reverse TTGGCTAGGCGACTGCTGGT
RPL27 mRNA forward GACGCAAAGCTGTCATCGTG
RPL27 mRNA reverse GCAGTTTCTGGAAGAACCAC