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Tyrosine phosphatase inhibition alters many components of the network. Selected phosphorylation patterns in response to 20 nM EGF stimulation with (dashed) and without (solid) 15 min pretreatment with 1 mM Na3VO4 for (A) EGFR pY1045, pY1068, pY1148, and pY1173 and (B) Gab1 pY259, pY373, pY627, and pY659. (C) Phosphorylation levels for several peptides were significantly altered in the pretreated (white) condition compared with untreated (black) in the absence of EGF.
