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. 2016 Feb 1;113(11):3000–3005. doi: 10.1073/pnas.1518499113

Fig. 1.

Fig. 1.

Fusing CD4 to Lck increases Lck in DSMs. (A) Percent of Lck-associated CD4 in WCL from CD4WT and CD4T cell lines relative to CD4–Lck lines (100%) (mean ± SEM from four experiments). (B) Membrane fractionation of 58α−β− cells expressing the 5c.c7 TCR plus CD4WT, CD4T, or CD4–Lck. gMFI −background (Bkg) within each fraction is shown as a percent of the total gMFI (–Bkg) for each cell line (mean ± SEM for three experiments). Background subtraction was performed with the gMFI of fraction 1 from each cell line. (C) Raw CD4-associated Lck signal (gMFI from each fraction). Data are representative of three experiments. *P < 0.05, Mann–Whitney.