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TCR scanning of MHCII on SNs. 58α−β− cells expressing CD4–Lck and the (A) 5c.c7 or OT-II TCR, (B) OT-I or gBT TCR, or (C) OIa.OIIb or OIIa.OIb TCR were cultured with 5 × 105 T cell-depleted C57BL/6 SNs and blocked with anti-MHCII and anti-CD4 mAbs. Data are mean ± SEM of triplicate wells and representative of at least two experiments.
