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. 2016 Feb 1;113(11):3000–3005. doi: 10.1073/pnas.1518499113

Fig. S6.

Fig. S6.

In vitro-generated 2a.OIIb and OIIa.2b TCRs display peptide-independent class II MHC specificity. Cell surface expression of TCR and CD4 were assessed by flow cytometry of 58α−β− cells expressing in vitro-generated TCRs made by pairing either (A) the TCR alpha subunit from the 2B4 TCR with the TCR beta subunit of the OT-II TCR (2a.OIIb) or (B) the TCR alpha subunit from the OT-II TCR with the TCR beta subunit of the 2B4 TCR (OIIa.2b). IL-2 secretion was detected by ELISA following 16 h of culture of 58α−β− cells expressing (C and E) 2a.OIIb or (D and F) OIIa.2b and the indicated CD4 molecule with M12 cells expressing the indicated peptide:MHC molecule. (G) IL-2 responses were blocked by addition of anti-MHC or anti-CD4 antibody (Cntrl, anti-CD8). Data are mean ± SEM of triplicate wells from one experiment.