Fig. S7.

Validation of microarray data by RT-qPCR. Expression of selected genes was assessed by RT-qPCR in leaf samples obtained from nonstressed and dehydrated Arabidopsis response regulator arr1,10,12 and WT plants to validate the microarray data. (A) Heatmap presentation of the fold-changes obtained from microarray analysis. (B) Heatmap presentation of the fold-changes obtained by RT-qPCR of three independent biological replicates. UBQ10 was used as reference gene. Changes in transcription abundance are indicated by intensities of colors expressed in log₂ scales with saturation at 6. Blue and red colors indicate down- and up-regulation, respectively. W-C, WT well-watered control; W-D2, WT dehydrated 2 h; W-D4, WT dehydrated 4 h; M-C, arr1,10,12 well-watered control; M-D2, arr1,10,12 dehydrated 2 h; M-D4, arr1,10,12 dehydrated 4 h.