Fig. 1.
Length, weight, adipose mass, and food intake in normal and leptin receptor mutant zebrafish. Heterozygous leprsa1508/+ mutant fish were crossed to provide WT (open bars) and homozygous leprsa1508/sa1508 (gray bars) offspring, which were grown together at similar densities, then characterized for growth, body composition, and feeding behavior, with the numbers (inside bars) and ages in days after fertilization (under bars) indicated. (A) Length of WT and leprsa1508/sa1508 mutant fish from 5 to 166 dpf. Two-way ANOVA shows a significant effect of age [F(5, 304)=1,284, P < 0.0001] but not genotype [F(1, 304) = 2.104, P > 0.05]. (B) Weight of WT and leprsa1508/sa1508 mutant fish from 100 to 166 dpf. Two-way ANOVA shows a significant effect of age [F(2, 151) = 227.1, P < 0.0001] but not genotype [F(1, 151) = 1.706, P > 0.05]. (C) Weight of WT and leprsa1508/sa1508 mutant fish following 30 d of high fat feeding. Two-way ANOVA shows significant increase in weight as a function of diet [F(1, 39) = 4.687 P < 0.05] but not of genotype [F(1, 39) = 0.03255, P > 0.05]. (D and E) Total triglyceride content of WT and leprsa1508/sa1508 mutant fish at 90 and 293 d of age, respectively. There was no significant difference at either age t (6) = 0.8631, P > 0.4213 (D) and t (18) = 0.9246, P > 0.3673 (E); two-tailed t test. (F and G) Food consumption of 3-mo-old male (F) and 1 y old female WT and leprsa1508/sa1508 mutant (G) fish over 16 trials. A repeated-measures ANOVA shows no significant effect of genotype in males [F(1, 127) = 0.00476, P > 0.05] or females [F(1, 319) = 1.013, P > 0.05]. Data shown as means ± SEM.