Fig. S1.

Protein alignments for predicted mutations. (A) Indicated in bold on the mouse leprb sequence are cytoplasmic amino acids 13–24 and 31–36, which have been shown to be essential for Jak2 recruitment and activation. Surrounded by a box are the amino acids comprising the transmembrane domain and the Box1 motif. (B) Alignment of zebrafish WT and Mutant lepa alleles tested for β-cell number. We identified two alleles in heterozygous F₁ fish that led to identical early stop codons between helix A and B of lepa. Stop codons are indicated with an asterisk; remaining C-terminal amino acids are indicated at the end of the sequence. AA, amino acid; DaRe, Danio rerio; MuMu, Mus musculus; OnMy, Oncorhynchus mykiss.