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Equimolar FID assays using 1–4 and thiazole orange as the intercalator probe. Conditions: [1–4] = 0.88 μM, [thiazole orange] = 1.25 μM, [base pairs] = 0.88 μM, 1 h, 24 °C. Mean percent displacement from three experiments and error bars representing 1 SD from the mean are shown.
