Fig. 4.

Dissection of the time course of LTM consolidation. (A) Most active period for consolidation occurred in the first 12 h after completion of spaced training. The 24-h LTM was tested without or with 2-min cold shock given at hours 2, 4, 8, 12, or 23, respectively, after training. (B) The time course of LTM consolidation was not altered by manipulation of DIM-7 expression. Overexpression of DIM-7 (UAS-msk/+; elav-GS/+, RU+) enhanced 24-h LTM, which was disrupted by cold shock at different time points (8 h, 12 h, or 23 h) after spaced training, but did not appear to speed up the time course. Bars, mean ± SEM (n = 8); *P < 0.05.