Table S1.
Strains and plasmids used in this study
| Name | Features | Source |
| Strains | ||
| Vibrio cholerae 2740–80 ΔClpVΔFlgGΔVipA | Streptomycin resistant, lacZ2 derivative of V. cholerae 2740-80 deleted for ClpV, FlgG and VipA genes | (3) |
| Escherichia coli DH5-α | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Life Technologies |
| Escherichia coli BL21-DE3 | hsdS gal (λcIts857 ind1 Sam7 nin5 lacUV5-T7 gene 1) | Life Technologies |
| Neisseria meningitidis NZ98/254 | ST-41/44 complex/lineage 3; B:4:P1.4 | (38) |
| Plasmids | ||
| pET-Duet | The vector encodes two multiple cloning sites with T7 promoter, pBR322-derived ColE1 replicon, lacI gene and AmpR | Novagen |
| pCOLA-Duet | The vector encodes two multiple cloning sites with T7 promoter, COLA replicon from ColA (1) and KanR | Novagen |
| pET-Duet-sfGFP-his | Construct to express recombinant sfGFP protein in E.coli | This study |
| pET-Duet-mCherry-his | Construct to express recombinant mCherry protein in E.coli | This study |
| pET-Duet-VipA-his | Construct to express recombinant V. cholerae VipA protein in E.coli | This study |
| pET-Duet-VipB-his | Construct to express recombinant V.cholerae VipB protein in E.coli | This study |
| pETDuet-VipAhis/VipA-sfGFP | Construct to coexpress V. cholerae VipAhis and VipA-sfGFP fusion protein in V. cholerae and E. coli | This study |
| pET-Duet-VipAhis/VipA-mCherry | Construct to coexpress V. cholerae VipAhis and VipA-mCherry fusion protein in E. coli | This study |
| pET-Duet-VipAhis/VipA-fHbp | Construct to coexpress V. cholerae VipAhis and VipA-fHbp fusion protein in E. coli | This study |
| pET-Duet-VipAhis/VipA-NHBA | Construct to coexpress V. cholerae VipAhis and VipA-NHBA fusion protein in E. coli | This study |
| pET-Duet-aVipAhis/aVipA-sfGFP | Construct to coexpress A. baylyi VipAhis and VipA-sfGFP fusion protein in E. coli | This study |
| pET-Duet-pVipAhis/pVipA-sfGFP | Construct to coexpress P. aeruginosa VipAhis and VipA-sfGFP fusion protein in E. coli | This study |
| pCOLA-Duet-VipAhis/VipB | Construct to coexpress V. cholerae VipAhis and VipB protein in V. cholerae and E. coli | This study |
| pCOLA-Duet-aVipAhis/aVipB | Construct to coexpress A. baylyi VipAhis and VipB protein in E. coli | This study |
| pCOLA-Duet-pVipAhis/pVipB | Construct to coexpress P. aeruginosa VipAhis and VipB protein in E. coli | This study |
| pET21b-fHbp-his | Construct to express recombinant N.meningitidis fHbp protein in E.coli | This study |
| pET21b-NHBA-his | Construct to express recombinant N.meningitidis NHBA protein in E.coli | This study |
| pTARA | pBAD33 plasmid carrying T7 RNA polymerase under the control of AraPBAD promoter | Addgene |