Fig 1. Western blot (a) and RT-PCR (b) of sodium glucose cotransporter 2.

The IgG antibody reacted with membrane proteins of the appropriate predicted molecular weight (73 kDa) from both mesangial cells (left) and renal proximal tubular epithelial cells (right). RT-PCR products for SGLT2 from the total RNA from both mesangial cells (A: left) and renal proximal tubular epithelial cells (B: right) revealed a single band of the appropriate predicted size (422 bp).