Table 1. Macro validation on high-content microscopy images.

NMJ feature	Average manual count	Macro count	Distribution (p-value)	Ccc	Average deviation	Sensitivity	Specificity
NMJ area	403μm2	392μm2	0.60	0.84	2.10%	x	x
Perimeter	257μm	269μm	0.38	0.95	5.10%	x	x
Boutons	25	16	<0.0001	0.22	35.02%
NMJ Length	111μm	117μm	0.41	0.96	4.55%	x	x
Longest branch length	96μm	102μm	0.34	0.94	6.11%	x	x
Islands	1.73	1.73	0.91	0.92	x	98.89%	99.17%
Branches	4.47	4.60	0.77	0.90	x	93.94%	97.30%
Branching points	1.33	1.53	0.49	0.91	x	91.39%	98.33%
Active zones	266	269	0.73	0.98	x	91.45%	92.64%