Table 2. Macro validation on confocal microscopy images.

NMJ feature	Manual count	Macro count	Significance (p-value)	Ccc	Average deviation	Sensitivity	Specificity
NMJ area	356 μm2	355 μm2	0.98	0.91	0.98%	x	x
Perimeter	247 μm	249 μm	0.93	0.92	1.99%	x	x
Boutons	22	16	0.01	0.55	27.47%
NMJ Length	108 μm	107 μm	0.96	0.99	0.30%	x	x
Longest branch length	99 μm	98 μm	0.96	0.99	0.14%	x	x
Islands	1.47	1.33	0.46	0.91	x	100%	93.33%
Branches	2.93	3.27	0.95	0.92	x	92.67%	96.00%
Branching points	0.67	1.00	0.60	0.83	x	92.78%	93.33%
Active zones	250	247	0.88	0.94	x	92.23%	91.26%