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. 2015 Aug 12;5(2):e1074376. doi: 10.1080/2162402X.2015.1074376

Figure 2.

Figure 2.

TLR4 promoted HCC proliferation in a STAT3 signaling pathway dependent manner. A. H7402 cells were treated with LPS and harvested at different time points. Then total proteins of these cells were extracted, and p-Tyr705-STAT3 and total STAT3 levels were detected by western blot. B. H7402 cells were co-transfected with pGL3-STAT3-TK-luciferase or pGL3-TK-luciferase and pRL-TK plasmid. After 12 h, cells were treated with LPS for 0.5 h, 1 h, 2 h or 6 h, and then the luciferase activity was measured. C. H7402 cells were transfected with STAT3-decoy ODN or scramble ODN with Lipofectamin™ 2000. After 12 h, these cells were treated with LPS for 24 h and 48 h, and WST-1 was used to assess the proliferation rate. D. H7402 cells were pre-treated with S3I-201 (100 μM) for 12 h, and then LPS was added into the culture. After 24 h, the proliferation rate was assessed by using WST-1. Values are means ± S.D. of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with untreated group.