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. 2015 Aug 12;5(2):e1074374. doi: 10.1080/2162402X.2015.1074374

Figure 3.

Figure 3.

Synergistic effects of dually delivered celecoxib (CXB) and anti-PD-1 mAb (αPD-1) on increasing the presence of INFγ-expressing CD4 and CD8 T cells. C57BL/6 mice received the treatments with the blank hydrogel (gel) and the hydrogels delivering CXB (+ CXB), anti-PD-1 mAb (+ αPD-1), or both (+ CXB + αPD-1) at Day 7 after the inoculation of 1.0 × 105 B16-F10 cells. (A and B) The representative flow cytometric analysis images (left) and the corresponding quantification (right) of IFN-γ positive CD4+ T cells (A) and CD8+ T cells (B) from the tumor draining lymph nodes of the mice 7 days after the treatments. (C and D) The similar T cell analyses for spleens. (E and F) The similar T cell analyses for tumor tissues. Each column represents 3 independent experiments (n = 6–8 mice per group per experiment). *P < 0.05, **P < 0.01, N.S., not significant, Student's t-tests. The asterisk or “N.S.” without a line underneath indicates the comparison to the blank hydrogel group. Error bars represent the standard error of the mean.