Figure 4.

161-Ab reduces tumor angiogenesis. RENCA and CT26 s.c. tumors were harvested and were formalin fixed and paraffin embedded. (A) Representative images of tissue sections that were immunohistochemically stained for CD31, the marker of endothelial cells; Vessel density was calculated using the Weibel grid applied to at least three different fields in each slide (n = 5). Bar size is 100 μm. (B) Tumor lysates were prepared, total protein concentrations were determined by the Bradford reagent and lysate concentrations of VEGF and MMP-9 were determined by ELISA (n = 4–5).