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. Author manuscript; available in PMC: 2016 Mar 21.
Published in final edited form as: Biochemistry. 2015 Oct 13;54(42):6545–6554. doi: 10.1021/acs.biochem.5b00876

Figure 2.

Figure 2

NMR evidence for a dynamic Rev/IA interface. (A) One-dimensional 1H NMR spectra of imino resonances from stems IIB (left) and IA (right) in free, Rev–ARM-bound or RevDO-bound state. (B) 1H–15N HSQC spectra of 15N-RevDO bound to stem IIB (left panel, blue) (shows ∼50 backbone amide resonances) and stem IA (middle panel, green) (shows ∼40 backbone amide resonances). The right panel shows overlay of spectra in the first two panels. Insets show overlays of the imino proton of Trp45 (observed only in the Rev/IIB spectrum, top left) and Hε protons of arginines from Rev/IIB (blue) and Rev/IA (green) (right).