Fig. 5.

Effect of RKS262 treatment on the expression profile of extended Bcl2 class proteins in OVCAR-3 cells. Expression of proapoptotic Bax, Bid, Bad, Bax, Bak and Boc and anti-apoptotic Bcl-xl and Mcl-1 in RKS262 (5 µM; for 1, 6, 18 and 36 h) and vehicle-treated OVCAR-3 cells were analyzed by Western blotting of lysates (Materials and Methods). As an internal standard for equal loading the blots were probed with an anti-β-actin antibody. Experiments were performed independently in duplicates showing identical outcomes