Table 3. Summary of virally encoded novel miRNA.

miRNA	Sequence(5’-3’)	Length	Strand and Position	Reads	RT-qPCR
prv-miR-1-5p	GGCGGTCGGGGGGCGCGTCGGGC	23	+3812–3834	478	-
prv-miR-2-3p	CATGCACCTGTACCTCTCGG	20	+8294–8313	136	+
prv-miR-3-3p	CGGCCAGCCCGGACGCGCTGTA	22	+10820–10841	5	+
prv-miR-4-3p	CGACGACTGGGGGCGCGCGCC	21	+60508–60528	16	+
prv-miR-5-3p	CGAGCTCTGCGACCGGCGCG	20	+60634–60653	10	+
prv-miR-6-5p	CGCAGGCGCGCGGCATGGAGGT	22	+60774–60795	10	-
prv-miR-7-5p	TACCCGGCGCCCGTGAACGCGCTC	24	+69940–69963	5	+
prv-miR-8-3p	CGAGCTCCTGCCGGCCCGCACG	22	+73963–73984	9	+
prv-miR-9-5p	TACGCGGCGCGCTTCGTCCACG	22	+85091–85112	6	+
prv-miR-10-5p	CCCGCGGACGCGCCGGAGGCGG	22	+86375–86396	105	+
prv-miR-11-5p	TCCACTTCTCGACGGCCGGCTCC	23	+100055–100077	28	+
prv-miR-12a/b-3p	CCAATCGGGTGGCAGCGGGGG	21	+102686-102706/-144463-144483	400	+
prv-miR-13a/b-5p	CCGGGGAAGGGTCGGGCGATG	21	+110905-110925/-136244-136264	983	+
prv-miR-14a/b-5p	CGCCTCGGGGCCGACGTGAACC	22	+117693-117714/-130124-130145	39	+
prv-miR-15-5p	TCTCCGCCGAGACGACCCCGGGC	23	+125184–125206	7	+
prv-miR-16-3p	CGCGGGCGGCGGGAGGGGAGGGA	23	+134599–134621	96	-
prv-miR-17a/b-5p	GCGACGGAAGGGGCAGGCTCGGCG	24	+144578-144601/-102568-102591	5635	+
prv-miR-18-3p	GGCCGGAACACCGAGCGATGG	21	-34759-34779	6	+
prv-miR-19-5p	ACGGAGCGCCTGGACGCCGCGGC	23	-36979-37001	103	+
prv-miR-20-3p	CCGTGCTGGCCGTGGTCGACGA	22	-57462-57483	114	+
prv-miR-21-3p	CACGCGGCGGGGGCGAGGGCGTCC	24	-86755-86778	7	-
prv-miR-22-3p	CCCGACGGGCTGGTGCGGACG	21	-87359-87379	105	+
prv-miR-23-5p	CTGGATCGTGTGCCTCGGCGTG	22	-89358-89379	11	+
prv-miR-24-3p	CGTTGAGGGTCTGGATGCACG	21	-90107-90127	34	+
prv-miR-25-5p	ACGGGCGCGCGGGCGGCGCCGG	22	-126341-126362	5	-

This table showed the name, sequences, length, genomic position and cloning frequency of novel miRNAs encoded by JS-2012 strain in this analysis. The qPCR results showed whether each PRV miRNA was detected (+) or not (-).