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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):255–269. doi: 10.1021/acs.chemrestox.5b00418

Figure 5.

Figure 5

TIC and MS2 spectra of [M+3H]3+ of peptide D (LH*CDKLHVDPE) adducts (A1) CID, (A2) HCD of β93Cys-SO-AαC at m/z 501.5664 at tR 13.26 min; (B1) CID, (B2) HCD of β93Cys-SO-ABP at m/z 496.9029 at tR 14.94 min; (C1) CID, (C2) HCD of β93Cys-SO-ANL at m/z 471.5591 at tR 12.26 min and (D) CID-MS3 of LHC[SO]DKLHVDPE [M′•]2+ at m/z 471.5591 >660.8101 > recovered from the Glu-C (1/25th enzyme to protein ratio) digest of Hb modified with 3 molar excess of respective HONH-AαC, HONH-ABP or HONH-ANL. MS/MS spectral data were acquired on Orbitrap MS at 30,000 resolving power.