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. Author manuscript; available in PMC: 2017 Mar 21.
Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):255–269. doi: 10.1021/acs.chemrestox.5b00418

Figure 9.

Figure 9

Ion counts of β93Cys-SO-ABP, β93Cys-SO2-ABP adducts and free ABP recovered from Glu-C digests of Hb from mouse treated with ABP (40 mg/kg body weight) and human Hb (2nmol) in vitro modified with 3 molar excess of HONH-ABP (6 nmol). The Glu-C digestion was carried out with Hb (5 μg of Hb from in vivo mice treated with ABP and Hb of modified in vitro with HONH-ABP). The protein digest (100 ng) was used for the LC-MS/MS based measurement of ABP-Hb adducts and free ABP. Data are plotted as mean and standard deviation of ion counts of adducts recovered from five ABP-treated mice and three independent experiments conducted in vitro. The level of adducts were determined from the area of total counts of CID-MS2 spectral data analyzed using orbitrap MS analyzer at 30,000 resolving power.