. Author manuscript; available in PMC: 2017 Mar 21.

Published in final edited form as: Chem Res Toxicol. 2016 Feb 22;29(3):255–269. doi: 10.1021/acs.chemrestox.5b00418

Table 1.

Determination of Free Cys and met-Hb Formation by N-Hydroxy Metabolites of AαC, ABP, PhIP, and MeIQx

Substrate	Reduced β-Cys⁹³	met-Hb (%)	mol met-Hb formed per mol of HONH-Substrate or H₂O^a
Hb + H₂O	2.00 ± 0.10	2.5 ± 0.14	0.03
Hb +HONH-MeIQx	1.96 ± 0.10	3.7 ± 0.18	0.05
Hb +HONH-PhIP	1.81 ± 0.10	4.7 ± 0.20	0.06
Hb +HONH-ABP	<0.10 ± 0.01	41.4 ± 0.33	0.55
Hb+HONH-AαC	<0.10 ± 0.02	48.8 ± 0.31	0.64

HbO₂ (tetramer) content of RBC was adjusted to 2 nmol and RBC were incubated with 6 nmol of HONH-AA or HONH-HAA for 30 min, followed by titration with PMB.

met-Hb formation in the presence or absence of HONH-AA or HONH-HAA (mol/mol); mol of met-Hb formation in the H₂O control was normalized to the ratio of the HONH-substrate treated cells by dividing amount of met-Hb formed by 6. Each value represents the mean and standard deviation of three experiments.