Figure 2.

Adhesion to fibronectin alters the IL-6-induced phosphotyrosine proteome of 8226 myeloma cells. 8226 myeloma cells were grown in suspension (Sus) or adhered to FN plates (FN) for 1 h prior to stimulation with 1 ng/ml IL-6 for 30 min. Lysates from 10⁸ cells per condition were digested with trypsin, and phosphotyrosine peptides were enriched by immunoprecipitation. Peptides are grouped according to changes in relative intensity between sample groups: phosphorylation induced by (a) suspension, (b) adhesion to FN, (c) Sus+IL-6 or (d) adhesion to FN+IL-6. Peptides with increasing relative abundance are indicated by blue shading; decreasing phosphorylation events are indicated by yellow shading. Peptides are identified by gene symbols.