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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Neurosci Res. 2016 Feb 11;94(5):401–408. doi: 10.1002/jnr.23719

Figure 1.

Figure 1

Calmodulin activity is required for mGluR1/5 signaling in primary hippocampal neurons. DIV (days in vitro) 8 mouse hippocampal neurons were treated with 100 uM DHPG for 10, 30 and 60 min (A and B). In C and D, hippocampal neurons were pre-treated with 75 uM W13 or vehicle control for 30 min, after which the cells were treated with 100 uM DHPG or vehicle for 30 min. The levels of pERK1/2 (A and C) and pS6K1 (at Thr421/Ser424) (B and D) were determined by Western blot. Representative images are shown in the upper panels and quantifications are shown in the lower panels. The level of pERK1/2 and pS6K1 was normalized to the level of total ERK1/2 and total S6K1, respectively. All data were collected from 6 independent samples. *: p < 0.05 between control and the indicated group. #: p < 0.05 between the indicated group and the DHPG-treated group. The p value was determined by one-way ANOVA followed by post hoc analysis.