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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Neurosci Res. 2016 Feb 11;94(5):401–408. doi: 10.1002/jnr.23719

Figure 3.

Figure 3

Inhibition of ERK1/2 suppresses mGluR1/5-mediated Arc up-regulation and synaptic depression. (A and B) Primary hippocampal neurons were treated with 10 uM MEK inhibitor U0126 for 30 min, following which the cells were stimulated with 100 uM DHPG for 30min. Protein samples were analyzed by Western blot and are presented as normalized protein levels relative to the no treatment control. (A) U0126 suppressed ERK1/2 phosphorylation in both control and DHPG-stimulated neurons. (B) U0126 inhibited DHPG-stimulated up-regulation of Arc protein expression. All data were collected from 6 independent samples. *: p < 0.05 between control and the indicated group. #: p < 0.05 between the indicated group and the DHPG-treated group. The p value was determined by one-way ANOVA followed by post hoc analysis. (C) U0126 suppressed DHPG-induced in vivo synaptic depression at CA1 synapses in anesthetized mice. 0.5 ul DHPG (2.5 mM, n=7) or 0.5 ul DHPG (2.5 mM) plus U0126 (2 mM) (n=6) was infused to the CA1 region. The relative fEPSP value was normalized to the baseline level.