TABLE 1.

Antibody Characterization

Antigen	Description of Immunogen	Source, Host Species, Cat. No., Lot No., RRID	WB dilution
Phosphorylated ERK1/2 at Thr202/Tyr204	synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase	Cell Signaling, rabbit, polyclonal, catalog No. 9101L, lot No. 28, RRID:AB_331647	1:1000
ERK1/2	synthetic peptide corresponding to a sequence in the C-terminus of rat p44 MAP Kinase	Cell Signaling, rabbit, polyclonal catalog No. 9102L, lot No. 23, RRID:AB_823494	1:1000
Phosphorylated	synthetic phosphopeptide corresponding to	Cell Signaling, rabbit, polyclonal,	1:1000
S6K1 at	residues surrounding Thr421/Ser424 of	catalog No. 9204L, lot No. 7,
Thr421/Ser424	human p70 S6 kinase	RRID:AB_2265913
S6K1	synthetic peptide corresponding to residues surrounding the carboxy-terminus of human p70 S6 kinase	Cell Signaling, rabbit, polyclonal, catalog No. 9202L, lot No. 8, RRID:AB_823592	1:1000
Arc	Strep-Tag® fusion protein of full-length mouse Arc	Synaptic Systems, rabbit, polyclonal catalog No. 156003, RRID:AB_887694)	1:1000
β-actin	synthetic peptide corresponding to the N- terminus of β-cytoplasmic actin (Ac-Asp- Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn- Gly-Ser-Gly-Lys, conjugated to KLH).	Sigma, mouse, monoclonal, catalog No. A5441, lot No. 122M4782,	1:10,000