Table 4.

Frequency of micronuclei, lobed, blebbed and notched nuclei and binucleated cells in peripheral blood of Astyanax gr. bimaculatus and Aequidens metae sampled in different sites of Ocoa River and a reference site (Negro River), Villavicencio—Meta, Colombia during dry and rainy season

Specie	Nuclear alteration	Dry				Rainy
		Site 1	Site 2	Site 3	Reference	Site 1	Site 2	Site 3	Reference
Astyanax gr. bimaculatus	MN	0.07 ± 0.01	0.28 ± 0.03*	0.35 ± 0.04*	0.12 ± 0.01	0.09 ± 0.01	0.27 ± 0.03*	0.36 ± 0.03*	0.09 ± 0.02
	Lobed	0.09 ± 0.02	0.37 ± 0.04*	0.44 ± 0.04*	0.09 ± 0.02	0.09 ± 0.01	0.42 ± 0.4*	0.51 ± 0.04*	0.11 ± 0.02
	Blebbed	0.11 ± 0.02	0.40 ± 0.03*	0.48 ± 0.04*	0.05 ± 0.01	0.08 ± 0.01	0.36 ± 0.04*	0.41 ± 0.04*	0.10 ± 0.01
	Notched	0.10 ± 0.02	0.66 ± 0.04*	0.71 ± 0.05*	0.07 ± 0.01	0.08 ± 0.01	0.73 ± 0.03*	0.74 ± 0.03*	0.11 ± 0.02
	Binucleated cell	0.08 ± 0.01	0.44 ± 0.04*	0.46 ± 0.05*	0.08 ± 0.01	0.07 ± 0.01	0.47 ± 0.04*	0.50 ± 0.05*	0.12 ± 0.01
Aequidens metae	MN	–	0.33 ± 0.02*	0.39 ± 0.01*	0.06 ± 0.01	–	0.36 ± 0.03*	0.37 ± 0.03*	0.09 ± 0.02
	Lobed	–	0.32 ± 0.02*	0.42 ± 0.02*	0.08 ± 0.01	–	0.41 ± 0.03*	0.44 ± 0.02*	0.09 ± 0.01
	Blebbed	–	0.34 ± 0.03*	0.41 ± 0.03*	0.07 ± 0.01	–	0.35 ± 0.03*	0.40 ± 0.01*	0.11 ± 0.02
	Notched	–	0.59 ± 0.03*	0.71 ± 0.03*	0.09 ± 0.02	–	0.74 ± 0.02*	0.70 ± 0.04*	0.08 ± 0.02
	Binucleated cell	–	0.38 ± 0.02*	0.40 ± 0.03*	0.12 ± 0.02	–	0.47 ± 0.04*	0.49 ± 0.03*	0.15 ± 0.03

* For the same season of year, asterisks indicate statistically significant differences (p < 0.05) between the reference site and the monitoring sites. Site 1 (before entering the city), site 2 (where sewage from the city is dumped), site 3 (after the city and close to a landfill)