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. 2016 Mar 22;7:101. doi: 10.3389/fimmu.2016.00101

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Copyright © 2016 Nielsen, Wolf, Goodier and Riley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antibody and IL-18 synergism in response to H3N2. PBMCs were stimulated for 18 h in vitro Med (medium alone), 5, 10, and 50 ng/ml IL-18 with or without 1 μg/ml inactivated influenza virus H3N2. Responses were measured as the percentage of NK cells expressing surface CD107a (A), surface CD25 (B), or intracellular IFN-γ (C) (n = 7–8, data from one experiment). Box plots show the 5th to 95th percentile range. Data were analyzed with paired Wilcoxon signed-rank tests. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.