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. 2016 Mar 22;10:73. doi: 10.3389/fncel.2016.00073

Figure 3.

Figure 3

Effect of the ablation of Sirt3 on gut function in healthy animals. (A) Contractile responses of longitudinal segments of colon driven by bethanechol (BCH, 10 μM) or electrical field stimulation (EFS) are similar in mice with wild type Sirt3 (wt) and Sirt3 null mice (Sirt3−/−; n = 5 animals per group, P > 0.05, Student’s t-test). (B) Relaxations driven by EFS (at 20 Hz) in pre-contracted (PGF2α, 5 μM) longitudinally-oriented segments of colon from Sirt3−/− mice and their respective wt controls. EFS produced significantly smaller relaxations in tissues from Sirt3−/− mice than wt controls (n = 5 animals per group, *P = 0.0192, Student’s t-test). Distal colonic transport time (C), the number of fecal pellets produced per hour (D) and pellet composition (E,F) are comparable between male and female agouti wt and Sirt3−/− mice (n = 4 animals per group; P > 0.05, 2-way ANOVA).