Figure 6.

Enteric neuron survival and glial reactivity in healthy and inflamed wt and Sirt3^−/− mice. (A–D”) show dual-label fluorescence immunohistochemistry for HuC/D (cyan, A–D) and GFAP (grayscale, A’–D’) in myenteric ganglia from saline- (A–A”, C–C”) or DNBS-treated (B–B”, D–D”) wt (A–B”) or Sirt3^−/− (C–D”) mice. Overlays are shown in (A”–D”). Scale bar in D” = 50 μm and applies to all images. (E) Quantification of myenteric neuron packing density. (F) Quantification of ganglionic GFAP fluorescence density (afu/μm²). n = 3–5 animals per group, *P < 0.05, **P < 0.01, ***P < 0.001, 2-way ANOVA.